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miR‐1, regulated by LMP1, suppresses tumour growth and metastasis by targeting K‐ras in nasopharyngeal carcinoma
Author(s) -
Chen Xi,
Shi Jingxuan,
Zhong Jianwen,
Huang Zhenyun,
Luo Xi,
Huang Yaping,
Feng Shuang,
Shao Jianbo,
Liu Dabo
Publication year - 2015
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12162
Subject(s) - nasopharyngeal carcinoma , downregulation and upregulation , cancer research , metastasis , epstein–barr virus , biology , biomarker , in vivo , in vitro , virus , microrna , carcinoma , cell growth , cancer , immunology , medicine , gene , radiation therapy , genetics
Summary There is evidence to show that downregulation of miR‐1 expression is closely related to cancer progression, including in nasopharyngeal carcinoma ( NPC ). However, the molecular mechanisms underlying miR‐1 downregulation in NPC remain largely unknown, especially its association with Epstein–Barr virus ( EBV ). In this study we found that restoration of miR‐1 dramatically inhibited cell invasion in vitro , together with tumour growth and metastasis in vivo . Importantly, we found that LMP 1, an Epstein–Barr virus ( EBV )‐associated protein, suppressed miR‐1 expression. Furthermore, we identified K‐ras as a novel direct target of miR‐1. Our results demonstrated for the first time that miR‐1 was suppressed by LMP 1 and its tumour‐suppressive effects were mediated chiefly by repressing K‐ras expression. We propose that miR‐1 could serve as an independent biomarker to identify patients with different clinical characteristics.