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Expression and significance of cortactin and HDAC 6 in human prostatic foamy gland carcinoma
Author(s) -
Hou Huilian,
Zhao Le,
Chen Wei,
Li Jing,
Zuo Qinqin,
Zhang Guanjun,
Zhang Xuebin,
Li Xu
Publication year - 2015
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12132
Subject(s) - cortactin , immunohistochemistry , cancer research , biology , pathology , cancer , prostate cancer , medicine , cell , cytoskeleton , genetics
Summary Cortactin, the cytoplasmic substrate of HDAC6, is known to play an actin cytoskeletal regulatory role which is implicated in the motility of cancer cells, and thus in cancer progression. Its activity is found to be regulated by HDAC 6. However, the significance of cortactin and HDAC 6 remains unclear in uncommon histologic variant human prostatic foamy gland carcinoma (PfCa). In this study, we aimed to identify the expression and potential role of cortactin and HDAC 6 in PfCa. Therefore, 16 PfCa specimens containing 48 foci with distinctive lesions were collected to identify the status of cortactin and HDAC 6 by immunohistochemistry. Their correlation between clinicopathological characteristics and prognostic values were further analysed. The effect of cortactin and HDAC 6 on prostate cancer cell migration and invasion was then evaluated in IA 8 cells. The results showed that expression of cortactin and HDAC 6 was significantly higher in PfCa foci, compared to that of high‐grade prostatic intraepithelial neoplasia ( HGPIN ) foci and benign foci ( P < 0.05). Cortactin and HDAC 6 were associated with poor prognosis of patients with PfCa ( P < 0.05). Multivariable Cox regression analysis showed HDAC 6 level was a significant prognostic factor for survival of patients with PfCa (β = 1.200, Wald value = 7.282, P = 0.007, 95% CI = 1.389–7.941, P < 0.01, β > 0). Both knocking down cortactin and inhibition of HDAC 6 activity with tubacin reduced in vitro migration and invasion ability of IA 8 cells substantially. Furthermore, HDAC 6 has prognostic value for patients with PfCa. Dysregulation of cortactin and HDAC 6 is implicated in the invasiveness and migration of prostate cancer cells.