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Oral administration of dextran sodium sulphate induces a caecum‐localized colitis in rabbits
Author(s) -
Leonardi Irina,
Nicholls Flora,
Atrott Kirstin,
Cee Alexandra,
Tewes Bernhard,
Greinwald Roland,
Rogler Gerhard,
FreyWagner Isabelle
Publication year - 2015
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12117
Subject(s) - lamina propria , colitis , caecum , histopathology , inflammatory bowel disease , cecum , crypt , inflammation , pathology , biology , medicine , immunology , epithelium , disease
Summary Trichuris suis ova ( TSO ) have shown promising results in the treatment of inflammatory bowel disease ( IBD ) but the mechanisms which underlies this therapeutic effect cannot be studied in mice and rats as T. suis fails to colonize the rodent intestine, whilst hatching in humans and rabbits. As a suitable rabbit IBD model is currently not available, we developed a rabbit colitis model by administration of dextran sodium sulphate ( DSS ). White Himalayan rabbits ( n = 12) received 0.1% DSS in the daily water supply for five days. Clinical symptoms were monitored daily, and rabbits were sacrificed at different time points. A genomewide expression analysis was performed with RNA isolated from caecal lamina propria mononuclear cells ( LPMC ) and intestinal epithelial cells ( IEC ). The disease activity index of DSS rabbits increased up to 2.1 ± 0.4 ( n = 6) at day 10 (controls <0.5). DSS induced a caecum‐localized pathology with crypt architectural distortion, stunted villous surface and inflammatory infiltrate in the lamina propria. The histopathology score reached a peak of 14.2 ± 4.9 ( n = 4) at day 10 (controls 7.7 ± 0.9, n = 5). Expression profiling revealed an enrichment of IBD ‐related genes in both LPMC and IEC . Innate inflammatory response, Th17 signalling and chemotaxis were among the pathways affected significantly. We describe a reproducible and reliable rabbit model of DSS colitis. Localization of the inflammation in the caecum and its similarities to IBD make this model particularly suitable to study TSO therapy in vivo .