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Comparative study of Hsp 27, GSK 3β, Wnt 1 and PRDX 3 in Hirschsprung's disease
Author(s) -
Gao Hong,
Liu Xiaomei,
Chen Dong,
Lv Liangying,
Wu Mei,
Mi Jie,
Wang Weilin
Publication year - 2014
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12075
Subject(s) - messenger rna , downregulation and upregulation , wnt signaling pathway , microbiology and biotechnology , pathology , hirschsprung's disease , blot , real time polymerase chain reaction , biology , western blot , immunohistochemistry , chemistry , medicine , disease , signal transduction , biochemistry , gene
Summary Hirschsprung's disease ( HSCR ) is a developmental disorder of the enteric nervous system characterized by aganglionosis in distal gut. In this study, we used two‐dimensional gel electrophoresis (2‐ DE ) technology coupled with matrix assisted laser desorption/ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF ‐ MS ) analysis to identify differentially expressed proteins in the aganglionic (stenotic) and ganglionic (normal) colon segment tissues from patients with HSCR . We identified 15 proteins with different expression levels between the stenotic and the normal colon segment tissues from patients with HSCR . Nine proteins were upregulated and six proteins downregulated in the stenotic colon segment tissues compared to the normal colon segment tissues. Based on the biological functions, we selected the Hsp 27 upregulated proteins and the PRDX 3 downregulated proteins to confirm their expression in 20 patients. The protein and mRNA expressions of Hsp 27 were statistically higher in the stenotic colon segment tissues than in the normal colon segment tissues, whereas the protein and mRNA expressions of PRDX 3 were statistically lower in the stenotic colon segment tissues than in the normal colon segment tissues. These findings of changes in mRNA and protein in tissues from patients with HSCR provide information which may be helpful in understanding the pathomechanism that is implicated in the disease.