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Changes in liver gluconeogenesis during the development of Walker‐256 tumour in rats
Author(s) -
Moreira Carolina Campos Lima,
Cassolla Priscila,
Dornellas Ana Paula Segantini,
Morais Hely,
Souza Camila Oliveira,
BorbaMurad Glaucia Regina,
Bazotte Roberto Barbosa,
Souza Helenir Medri
Publication year - 2013
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12002
Subject(s) - gluconeogenesis , medicine , endocrinology , glycerol , alanine , biology , urea , metabolism , pyruvate carboxylase , pyruvic acid , biochemistry , chemistry , enzyme , amino acid
Summary Few studies have investigated liver gluconeogenesis in cancer and there is no agreement as to whether the activity of this pathway is increased or decreased in this disease. The aim of this study was to evaluate gluconeogenesis from alanine, pyruvate and glycerol, and related metabolic parameters in perfused liver from W alker‐256 tumour‐bearing rats on days 5 ( WK 5 group), 8 ( WK 8 group) and 12 ( WK 12 group) of tumour development. There was reduction ( P < 0.05) of liver glucose production from alanine and pyruvate in WK 5, WK 8 and WK 12 groups, which was accompanied by a decrease ( P < 0.05) in oxygen consumption. Moreover, there was higher ( P < 0.05) pyruvate and lactate production from alanine in the WK 5 group and a marked reduction ( P < 0.05) of pyruvate and urea production from alanine in the WK 12 group. In addition, liver glucose production and oxygen consumption from glycerol were not reduced in WK 5, WK 8 and WK 12 groups. Thus the, the results show inhibition of hepatic gluconeogenesis from alanine and pyruvate, but not from glycerol, on days 5, 8 and 12 of Walker‐256 tumour development, which can be attributed to the metabolic step in which the substrate enters the gluconeogenic pathway.