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IgG4‐positive plasma cells are more often detected in chronic periapical lesions arising from permanent rather than primary teeth
Author(s) -
Polanco X. B. J.,
Bertasso A. S.,
Silveira H. A.,
Yamamoto de Almeida L.,
Almeida L. K. Y.,
Silva R. A. B.,
Silva L. A. B.,
Rossi A.,
NelsonFilho P.,
León J. E.
Publication year - 2021
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.13464
Subject(s) - immunoglobulin d , immunoglobulin light chain , pathology , plasma cell , medicine , permanent teeth , radicular cyst , antibody , immunohistochemistry , eosinophil , adenoid , dentistry , b cell , odontogenic , bone marrow , immunology , asthma
Aim To characterize plasma cell subsets in chronic periapical lesions affecting permanent and primary teeth. Methodology Only chronic periapical lesions without root canal treatment were selected. Twenty‐one radicular cysts and 7 periapical granulomas affecting permanent teeth and 19 radicular cysts and 4 periapical granulomas affecting primary teeth were assessed for immunoglobulin (Ig) light chain (kappa and lambda), Ig heavy chain (IgG, IgG4, IgA, IgM and IgD) and plasma cell immunohistochemical markers (MUM1/IRF4, EMA and CD138). The data acquired were analysed by Student’s t test, Mann–Whitney U, Friedman test followed by Dunn's multiple comparison test and Spearman’s rank correlation. Results All cases were polyclonal (having similar kappa/lambda light chain ratios). IgG was most abundant compared to other Ig heavy chains (all, P < 0.001); like Ig light chains, but unlike IgA, there was greater expression of IgG in the primary compared to the permanent dentition, for both radicular cysts ( P < 0.001) and periapical granulomas ( P = 0.53 ). Notably, IgG4 expression was greater in the permanent than the primary dentition, for both radicular cyst ( P < 0.05) and periapical granuloma ( P = 0.65 ). IgM and IgD expression was scarce and variable, whereas plasma cell populations were detected efficiently through EMA, CD138 and MUM1/IRF4 markers, the latter being more sensitive in both dentitions. Conclusions There were slight variations in the Ig light and heavy chain profiles in chronic periapical lesions when comparing the permanent and primary dentitions. The ability of IgG4+ plasma cell infiltration to modulate inflammatory responses in chronic periapical lesions arising from permanent as opposed to primary teeth should be considered in future studies.