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Electrokinetic transport and distribution of antibacterial nanoparticles for endodontic disinfection
Author(s) -
Ionescu A.,
Harris D.,
Selvaganapathy P. R.,
Kishen A.
Publication year - 2020
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.13321
Subject(s) - root canal , electrokinetic phenomena , zeta potential , materials science , biomedical engineering , nanoparticle , electrophoresis , biofilm , enterococcus faecalis , nanotechnology , dentistry , chemistry , chromatography , bacteria , medicine , biochemistry , escherichia coli , biology , gene , genetics
Aim To assess a novel, noninvasive intervention capable of mobilizing charged antibacterial nanoparticles to the apical portions of the root canal system, utilizing the principles of electrokinetics. Methods Experiments were conducted in three stages. Stage‐1: A computer model was generated to predict and visualize the electric field and current density distribution generated by the proposed intervention. Stage‐2: Transport of chitosan nanoparticles (CSnp) was evaluated qualitatively using a transparent microfluidic model with fluorescent‐labelled CSnp. Stage‐3: An ex vivo model was utilized to study the antimicrobial efficacy of the proposed treatment against 3‐week‐old monospecies E. faecalis biofilms. Scanning electron microscopy (SEM) was also utilized in this stage to confirm the deposition of CSnp. Results The results of the computer simulations predicted an electric field and current density that reach their maxima at the apical constriction of the root canal. Correspondingly, the microfluidic experiments demonstrated rapid, controlled CSnp transport throughout the simulated root canal anatomy with subsequent distribution and deposition in the apical constriction as well as periapical regions. Infected root canals when subjected to the novel treatment method resulted in a mean bacterial reduction of 2.1 log CFU. SEM analysis revealed electrophoretic deposition of chitosan nanoparticles onto the root canal dentine walls in the apical region. Conclusion The findings from this study demonstrate that the combination of cationic antibacterial nanoparticles with a low‐intensity electric field results in particle transportation (electrophoresis) and deposition within the root canal. This results in a synergistic antibiofilm efficacy and has the potential to enhance root canal disinfection.