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The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching
Author(s) -
Benetti F.,
Briso A. L. F.,
Carminatti M.,
Araújo Lopes J. M.,
Barbosa J. G.,
Ervolino E.,
GomesFilho J. E.,
Cintra L. T. A.
Publication year - 2019
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.13049
Subject(s) - osteopontin , osteocalcin , pulp (tooth) , inflammation , pulp capping , dentistry , chemistry , molar , coronal plane , andrology , medicine , anatomy , alkaline phosphatase , biochemistry , enzyme
Aim To analyse the influence of H 2 O 2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species ( ROS ) antibody. Methodology The maxillary molars of 50 rats were treated with 35% H 2 O 2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days ( n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS ‐positive cells were counted. Paired t ‐test and Wilcoxon signed‐rank test were used ( P < 0.05). Results The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls ( P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods ( P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching ( P < 0.05). The Ble group had more ROS ‐positive cells in the pulp at 7 and 15 days ( P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter ( P < 0.05). Conclusions Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ ROS was involved in cellular defence against H 2 O 2 .