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The potential application of concentrated growth factor in regenerative endodontics
Author(s) -
Hong S.,
Li L.,
Cai W.,
Jiang B.
Publication year - 2019
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.13045
Subject(s) - alkaline phosphatase , growth factor , chemistry , cell growth , vascular endothelial growth factor , andrology , microbiology and biotechnology , pulp (tooth) , platelet derived growth factor receptor , biology , pathology , biochemistry , medicine , enzyme , cancer research , vegf receptors , receptor
Aim To investigate the potential application of concentrated growth factor ( CGF ) to promote pulp regeneration within immature teeth. Methodology Concentrated growth factor clots produced from peripheral blood samples were investigated histologically by haematoxylin–eosin ( HE ) staining and evaluated morphologically by scanning electron microscope ( SEM ). The cytokines were extracted from the CGF , and representative cytokines were quantified by enzyme‐linked immunosorbent assay ( ELISA ). The biological effects of the CGF on human stem cells from the apical papilla ( SCAP s) were then investigated and quantified, including cell proliferation, cell migration, mineralized nodule formation, and the gene expression of alkaline phosphatase ( ALP ), dentine sialophosphoprotein ( DSPP ) and dentine matrix protein ( DMP )‐1. The results were analysed statistically using one‐way analysis of variance ( anova ). Results Concentrated growth factor had a complex three‐dimensional structure with a high density of platelets and nucleated cells. Representative growth factors including PDGF ‐ BB , IGF ‐1, TGF ‐β1, bFGF and VEGF were detected. The growth rate and migratory cell numbers of the CGF groups were significantly greater than those in the control groups ( P < 0.05). The mineralization areas in the CGF groups were significantly larger than those in the control groups ( P < 0.05). The expression levels of ALP , DSPP and DMP ‐1 were significantly up‐regulated after induction by CGF ( P < 0.05). Conclusions Concentrated growth factor promoted the proliferation, migration and differentiation of SCAP s and could be a promising biomaterial applied in regenerative endodontics.