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Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation
Author(s) -
Morgan A. D.,
Ng Y.L.,
Odlyha M.,
Gulabivala K.,
Bozec L.
Publication year - 2019
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.13004
Subject(s) - sodium hypochlorite , fourier transform infrared spectroscopy , dentinal tubule , root canal , ex vivo , materials science , chemistry , dentin , smear layer , spectroscopy , nuclear chemistry , dentistry , composite material , chemical engineering , biochemistry , in vitro , medicine , organic chemistry , physics , quantum mechanics , engineering
Abstract Aim To establish a method using Fourier Transform Infra‐Red spectroscopy ( FTIR ) to characterize the nature and depth of changes in dentinal collagen following exposure to sodium hypochlorite (Na OC l) during root canal irrigation in an ex vivo model. Methodology Fourier Transform Infra‐Red spectroscopy was used to assess the changes in dentinal collagen when the root canal was exposed to Na OC l. The changes in dentinal collagen caused by Na OC l irrigation of root canals in transverse sections of roots, at 0.5 mm from the canal wall and 0.5 mm from the external root surface, were assessed by FTIR . The data were analysed using paired t ‐test with 5% significance level. Results Fourier Transform Infra‐Red spectroscopy confirmed that Na OC l exposure caused alterations in the chemistry and structure of collagen in dentine. FTIR spectra obtained from dentine surfaces and dentine adjacent to root canals exposed to Na OC l, all consistently showed degradation and conformational change of the collagen structure. FTIR data from the ex vivo model showed that the depth of effect of Na OC l extended to at least 0.5 mm from the canal wall. Conclusion In extracted human teeth, Na OC l caused changes in dentinal collagen that were measurable by FTIR . In an ex vivo model, the depth of effect into dentine extended at least 0.5 mm from the canal wall.