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Influence of dentine debris and organic tissue on the properties of sodium hypochlorite solutions
Author(s) -
Tejada S.,
Baca P.,
FerrerLuque C. M.,
RuizLinares M.,
Valderrama M. J.,
AriasMoliz M. T.
Publication year - 2019
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12986
Subject(s) - sodium hypochlorite , biofilm , chlorine , dissolution , chemistry , enterococcus faecalis , dentin , titration , nuclear chemistry , dentistry , inorganic chemistry , biochemistry , bacteria , medicine , biology , organic chemistry , genetics , escherichia coli , gene
Aim To determine the free available chlorine of 2.5% sodium hypochlorite (Na OC l) alone and combined with 9% etidronic acid ( HEDP ) in the presence of inhibitors, organic tissue and organic tissue plus dentine debris; to evaluate the influence of dentine debris on the tissue‐dissolving capacity of both Na OC l solutions; and to determine the antimicrobial action of these solutions when in contact with organic tissue and organic tissue plus dentine debris. Methodology The available chlorine of the solutions over time in the absence and presence of the inhibitors was measured using a titration method. The organic tissue dissolution by the solutions alone and in the presence of dentine powder was evaluated by weighing bovine tissue specimens before and after exposure to the solutions for 3 and 10 min. For the antimicrobial activity, biofilms of Enterococcus faecalis were exposed to the solutions for 3 min in the absence and presence of organic tissue and organic tissue + dentine debris. The biovolume and percentage of damaged membrane cells of the biofilm were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences ( P < 0.05). Results Both inhibitors consumed the free available chlorine of the solutions over time. The presence of dentine debris significantly reduced the tissue dissolution capacity of the Na OC l solutions ( P < 0.05). The percentages of biovolume reduction were not affected by the presence of the inhibitors in the two Na OC l solutions, whereas the percentage of damaged membrane cells was significantly reduced ( P < 0.001). Overall, a similar behaviour was observed in the Na OC l and Na OC l/ HEDP groups. Conclusions The presence of organic tissue and organic tissue + dentine debris favoured rapid consumption of the free chlorine of Na OC l and Na OC l/ HEDP . This resulted in a decreased ability to dissolve organic tissue without affecting the short‐term antimicrobial activity.