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Activation of α SMA expressing perivascular cells during reactionary dentinogenesis
Author(s) -
VidovicZdrilic I.,
Vijaykumar A.,
Mina M.
Publication year - 2019
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12983
Subject(s) - odontoblast , dentinogenesis , dentinogenesis imperfecta , pulp (tooth) , sma* , reactionary , anatomy , dental pulp stem cells , microbiology and biotechnology , biology , pathology , stem cell , medicine , combinatorics , politics , law , osteogenesis imperfecta , mathematics , political science
Aim To examine the contribution of perivascular cells expressing α SMA to reactionary dentinogenesis. Methodology An inducible, Cre‐loxP in vivo fate‐mapping approach was used to examine the contribution of the descendants of cells expressing the α SMA ‐Cre ERT 2 transgene to reactionary dentinogenesis in mice molars. Reactionary dentinogenesis was induced by experimental mild injury to dentine without pulp exposure. The Student's t test was used to determine statistical significance at * P ≤ 0.05. Results The lineage tracing experiments revealed that mild injury to dentine first led to activation of α SMA ‐tdTomato + cells in the entire pulp chamber. The percentage of areas occupied by α SMA ‐tdTomato + in injured (7.5 ± 0.7%) teeth were significantly higher than in teeth without injury (2 ± 0.5%). After their activation, α SMA ‐tdTomato + cells migrated towards the site of injury, gave rise to pulp cells and a few odontoblasts that became integrated into the existing odontoblast layer expressing Col2.3‐ GFP and Dspp . Conclusion Mild insult to dentine activated perivascular α SMA ‐tdTomato + cells giving rise to pulp cells as well as a few odontoblasts that were integrated into the pre‐existing odontoblast layer.