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Concentration‐dependent effect of bleaching agents on the immunolabelling of interleukin‐6, interleukin‐17 and CD5‐positive cells in the dental pulp
Author(s) -
Benetti F.,
GomesFilho J. E.,
Ferreira L. L.,
SivieriAraújo G.,
Ervolino E.,
Briso A. L. F.,
Cintra L. T. A.
Publication year - 2018
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12891
Subject(s) - pulp (tooth) , interleukin , molar , dentistry , interleukin 1β , inflammation , medicine , andrology , pathology , cytokine
Aim To evaluate lymphocyte‐like cell activation (CD5‐positive cells) and the expression of interleukin (IL)‐6 and IL‐17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H 2 O 2 ). Methodology The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20% H 2 O 2 (BLUE group, 1 application of 50 min), 35% H 2 O 2 (MAXX group, three applications of 15 min), or placebo gel (control). After 2 and 30 days, the rats were killed ( n = 10), and the jaws were processed for histological and immunohistochemistry analysis of the pulp tissue. The scores of inflammation and immunolabelling (IL‐6/IL‐17) were submitted to Mann–Whitney and Kruskal–Wallis followed Dunn tests, respectively; anova tests were used for comparisons of number of CD5‐positive cells and pulp chamber area values ( P < 0.05). Results At 2 days, 60% of specimens of the BLUE group were associated with moderate inflammation in pulp horns, and in the MAXX group with necrosis ( P < 0.05). At 30 days, the pulp was organized, and tertiary dentine was formed. The MAXX group had superior immunolabelling of IL‐17 at 2 days differing significantly from other groups ( P < 0.05). At 2 days, 90% of the specimens of the BLUE group had moderate immunolabelling of IL‐6, and 50% of the MAXX group had severe immunolabelling, both significantly different from the control ( P < 0.05). There was no significant difference between the groups at 30 days ( P > 0.05). CD5‐positive cells were present at 2 and 30 days, particularly in the bleached groups ( P < 0.05), without significant difference between time periods ( P > 0.05). Conclusions IL‐6 and IL‐17 participated in inflammation in the pulp tissue of rats after tooth bleaching, particularly at 2 days. The immunolabelling was greater with increasing H 2 O 2 concentration. This process was accompanied by the prolonged activation of CD5‐positive cells.