Cytocompatibility of Biodentine and iR oot FS with human periodontal ligament cells: an in vitro study

Aim To evaluate the cytocompatibility of Biodentine and iR oot FS with human periodontal ligament cells ( hPDLC s). Methodology Human periodontal ligament cells were characterized by flow cytometry and immunocytochemical analysis. Human periodontal ligament cell adhesion was assessed by scanning electron microscopy at day 3; proliferation by live/dead assay at days 1, 3 and 7; and osteogenic differentiation by alkaline phosphatase ( ALP ) activity staining, ALP quantification analysis and qRT ‐ PCR at days 7 and 14. Data were analysed with anova and independent sample t‐ tests with SPSS 21.0. Results Both iR oot FS and Biodentine increased the adhesion of hPDLC s at day 3. Compared to Biodentine, iR oot FS positively increased hPDLC proliferation on days 3 ( P  =   0.03) and 7 ( P  =   0.00). Osteogenic marker ALP was observed consistently in all samples, with iR oot FS having significantly higher ALP activity at day 14 ( P  =   0.00). Compared with Biodentine, iR oot FS significantly increased the mRNA level of ALP , COL 1 and Runx2 , and OCN increased only on day 14 ( P  < 0.05). Conclusions iR oot FS had a positive effect on the adhesion, proliferation and biomineralization of hPDLC s compared with Biodentine.