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Cytocompatibility of Biodentine and iR oot FS with human periodontal ligament cells: an in vitro study
Author(s) -
Luo T.,
Liu J.,
Sun Y.,
Shen Y.,
Zou L.
Publication year - 2018
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12889
Subject(s) - periodontal fiber , alkaline phosphatase , flow cytometry , staining , andrology , in vitro , adhesion , microbiology and biotechnology , chemistry , biology , dentistry , medicine , biochemistry , genetics , organic chemistry , enzyme
Aim To evaluate the cytocompatibility of Biodentine and iR oot FS with human periodontal ligament cells ( hPDLC s). Methodology Human periodontal ligament cells were characterized by flow cytometry and immunocytochemical analysis. Human periodontal ligament cell adhesion was assessed by scanning electron microscopy at day 3; proliferation by live/dead assay at days 1, 3 and 7; and osteogenic differentiation by alkaline phosphatase ( ALP ) activity staining, ALP quantification analysis and qRT ‐ PCR at days 7 and 14. Data were analysed with anova and independent sample t‐ tests with SPSS 21.0. Results Both iR oot FS and Biodentine increased the adhesion of hPDLC s at day 3. Compared to Biodentine, iR oot FS positively increased hPDLC proliferation on days 3 ( P = 0.03) and 7 ( P = 0.00). Osteogenic marker ALP was observed consistently in all samples, with iR oot FS having significantly higher ALP activity at day 14 ( P = 0.00). Compared with Biodentine, iR oot FS significantly increased the mRNA level of ALP , COL 1 and Runx2 , and OCN increased only on day 14 ( P < 0.05). Conclusions iR oot FS had a positive effect on the adhesion, proliferation and biomineralization of hPDLC s compared with Biodentine.