Premium
Response of porcine epithelial rests of Malassez to stimulation by interleukin‐6
Author(s) -
Sako R.,
Kobayashi F.,
Aida N.,
Furusawa M.,
Muramatsu T.
Publication year - 2018
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12862
Subject(s) - cell migration , stimulation , wound healing , cell growth , integrin , immunofluorescence , microbiology and biotechnology , cell culture , andrology , cell , chemistry , antibody , immunology , medicine , biology , biochemistry , genetics
Aim To investigate the proliferation and migration of epithelial cell rests of Malassez (ERM) after stimulation with IL‐6. Methodology Porcine‐derived ERM were seeded on Dulbecco's modified Eagle's Medium, and IL‐6 (100 pg mL −1 ) was incorporated into the culture medium. The WST‐1 assay was performed to evaluate cell proliferation, and absorption was measured at 450 nm. A wound‐healing assay and immunofluorescence assay for integrin α 3 were conducted to investigate migration. The Kruskal–Wallis test and the Mann–Whitney U ‐test with Bonferroni correction were used to analyse data of WST‐1 and wound‐healing assays. Results Cell proliferation following the stimulation by IL‐6 increased over time, with a significant increase being observed at 6 h ( P < 0.05), but not in a concentration‐dependent manner. Cell proliferation was significantly greater in IL‐6‐treated ERM than in nontreated ERM ( P < 0.05). The results of the wound‐healing assay revealed earlier closure in IL‐6‐treated ERM ( P < 0.05). In the immunofluorescence assay, integrin α 3 was detected at the edge of cell processes adjacent to the wound area. A neutralized antibody abrogated the effects of the IL‐6 stimulation in cell proliferation and migration. Conclusion IL‐6 promoted the proliferation and migration of porcine ERM in vitro .