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Biocompatibility and mineralized nodule formation of Neo MTA Plus and an experimental tricalcium silicate cement containing tantalum oxide
Author(s) -
TanomaruFilho M.,
Andrade A. S.,
Rodrigues E. M.,
Viola K. S.,
Faria G.,
Camilleri J.,
GuerreiroTanomaru J. M.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12780
Subject(s) - biocompatibility , serial dilution , cytotoxicity , mtt assay , nuclear chemistry , viability assay , alkaline phosphatase , chemistry , microbiology and biotechnology , mineralogy , biochemistry , biology , medicine , in vitro , pathology , organic chemistry , alternative medicine , enzyme
Aim To evaluate the biocompatibility and mineralized nodule formation of an experimental tricalcium silicate cement with tantalum oxide ( TSC /Ta 2 O 5 ) as radiopacifier, Neo MTA Plus (Avalon Biomed Inc., Bradenton, FL , USA ) and MTA (Angelus, Londrina, PR , Brazil) on human osteoblast‐like cells (Saos‐2). Methodology Biocompatibility was evaluated by 3‐(4,5‐dimethyl‐thiazoyl)‐2,5‐diphenyl‐tetrazolium bromide ( MTT ) and neutral red ( NR ) assays, after exposure of Saos‐2 to cement extracts at 1 : 1, 1 : 2, 1 : 4 and 1 : 8 dilutions for 24 h. Bioactivity was evaluated by alkaline phosphatase ( ALP ) activity, and calcium deposits were detected with alizarin red staining ( ARS ). Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05). Results The MTT assay revealed lower cytotoxicity for NEO and MTA ( P < 0.05), and higher for TSC /Ta 2 O 5 at 1 : 1 and 1 : 2 dilutions when compared to serum‐free medium – control ( P > 0.05). At 1 : 4 dilution, the TSC /Ta 2 O 5 cytotoxicity was similar to the control ( P > 0.05). At 1 : 8 dilution, cell viability was significantly greater than the control ( P < 0.05). Saos‐2 cell viability performed using the NR assay at all dilutions revealed no cytotoxic effect of MTA , NEO and TSC /Ta 2 O 5 . ALP activity at 1 and 3 days was similar to the control ( P > 0.05). TSC /Ta 2 O 5 had significantly greater ALP activity at 7 days when compared with the control ( P < 0.05). All materials induced the production of mineralized nodules, and NEO produced significantly more mineralized nodules than MTA and TSC /Ta 2 O 5 ( P < 0.05). Conclusions Neo MTA Plus and TSC /Ta 2 O 5 were biocompatible and induced ALP activity in Saos‐2 cells. Both materials induced mineralized nodule formation by Saos‐2 with Neo MTA Plus producing significantly more.