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Biocompatibility of three new calcium silicate‐based endodontic sealers on human periodontal ligament stem cells
Author(s) -
ColladoGonzález M.,
GarcíaBernal D.,
OñateSánchez R. E.,
OrtolaniSeltenerich P. S.,
Lozano A.,
Forner L.,
Llena C.,
RodríguezLozano F. J.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12703
Subject(s) - periodontal fiber , biocompatibility , viability assay , mesenchymal stem cell , periodontal ligament stem cells , dentistry , cell growth , flow cytometry , mtt assay , stem cell , materials science , chemistry , cell , biomedical engineering , medicine , microbiology and biotechnology , pathology , alkaline phosphatase , biology , biochemistry , immunology , metallurgy , enzyme
Aim To evaluate the biocompatibility of three calcium silicate‐based endodontic sealers, Bioroot BC Sealer (Septodont, Saint‐Maur‐des‐Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano‐ceramic Sealer (B&L Biotech, Fairfax, VA, USA) ( NCS ), on human periodontal ligament stem cells ( h PDLSC s). Methodology Human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound‐healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, h PDLSC s were directly seeded onto the material surfaces and analysed by scanning electron microscopy ( SEM ). One‐way analysis of variance ( anova ) followed by a Bonferroni post‐test was performed ( P < 0.05). Results At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer ( BR ) and Nano‐ceramic Sealer ( NCS ), but not Endoseal MTA ( ES ). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation ( P < 0.05). Similar results were obtained in a cell death assay. In addition, h PDLSC s maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR . Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs. Conclusions BR and NCS were associated with better cytocompatibility than ES . Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate‐based endodontic sealers for clinical application.