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The effect of dental bleaching on pulpal tissue response in a diabetic animal model
Author(s) -
Cintra L. T. A.,
Ferreira L. L.,
Benetti F.,
Gastélum A. A.,
GomesFilho J. E.,
Ervolino E.,
Briso A. L. F.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12692
Subject(s) - alloxan , haematoxylin , molar , diabetes mellitus , medicine , trichrome , masson's trichrome stain , inflammation , pulp (tooth) , necrosis , dentistry , h&e stain , endocrinology , fibrosis , immunohistochemistry
Aim To evaluate pulpal tissue response after dental bleaching in normal and alloxan‐induced diabetic rats. Methodology Twenty‐eight rats were divided into two groups of normoglycaemic and diabetic rats ( n = 14). Diabetes mellitus ( DM ) was induced with alloxan. After DM confirmation, all rats were anaesthetized and dental bleaching was performed with 35% hydrogen peroxide (H 2 O 2 ) on the right maxillary molars for 30 min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N‐bleached ( NB le), diabetic (D) and D‐bleached ( DB le). After 2 or 30 days, the animals were euthanized and the hemimaxillae were removed, processed for histopathological analysis and stained with haematoxylin–eosin ( HE ), Masson's trichrome ( MT ) and picrosirius red ( PSR ). Results obtained within animals (normoglycaemic or diabetic rats) were submitted to Wilcoxon or paired t ‐tests, and between animal (normoglycaemic and diabetic rats), to Mann–Whitney test or t ‐tests. Results At 2 days, the NB le group had a mild inflammatory infiltration in the pulpal tissue, whilst the DB le had severe inflammation or necrosis ( P < 0.05). At 30 days, no inflammation was present. However, a significant difference in pulp chamber area reduction by reactionary dentine deposition was found between the NB le and DB le groups ( P < 0.05). At 2 days, fewer immature collagen fibres and more mature collagen fibres were noted in the NB le, D and DB le groups; this was significantly different when compared to the N group ( P < 0.05). At 30 days, significantly fewer immature collagen fibres and more mature collagen fibres were noted in NB le compared with DB le group ( P < 0.05). Conclusions The inflammatory tissue response in rats’ teeth after dental bleaching was greater in diabetic rats. Additionally, the increase in reactionary dentine deposition and mature collagen fibres observed in diabetic rats needs further evaluation to confirm the present results.