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Human dental pulp cells response to mineral trioxide aggregate ( MTA ) and MTA Plus: cytotoxicity and gene expression analysis
Author(s) -
Rodrigues E. M.,
Cornélio A. L. G.,
Mestieri L. B.,
Fuentes A. S. C.,
Salles L. P.,
RossaJunior C.,
Faria G.,
GuerreiroTanomaru J. M.,
TanomaruFilho M.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12683
Subject(s) - mineral trioxide aggregate , alkaline phosphatase , viability assay , mtt assay , cytotoxicity , microbiology and biotechnology , chemistry , pulp capping , bone morphogenetic protein 2 , osteocalcin , andrology , pulp (tooth) , biology , apoptosis , biochemistry , in vitro , dentistry , medicine , enzyme
Aim To investigate the cytotoxicity, osteogenic bioactivity and mRNA expression of osteogenic markers of bone morphogenetic protein 2 ( BMP ‐2), osteocalcin ( OC ) and alkaline phosphatase ( ALP ) induced by the extracts of set MTA Plus ( MTA P) (Avalon Biomed Inc. Bradenton, FL , USA ) in comparison with MTA (Angelus, Londrina, PR , Brazil) on human dental pulp cells ( hDPC s). Methodology Cell viability was assessed by mitochondrial dehydrogenase enzymatic ( MTT ) assay, and the mechanism of cell death was evaluated by flow cytometry. Bioactivity was evaluated by alkaline phosphatase ( ALP ) assay and detection of calcium deposits with alizarin red staining ( ARS ). The gene expression of BMP ‐2, OC and ALP was quantified with real‐time PCR . Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05). Results MTA and MTA P were not cytotoxic and did not induce apoptosis. MTA P had significant higher ALP activity in relation to MTA and the control ( P < 0.05). MTA had a significantly higher percentage of mineralized area than MTA P ( P < 0.05). The expression of BMP 2 and OC mRNA was significantly higher in cells exposed to MTA than MTA P after 1 day ( P < 0.05). At day 3, the mRNA expression of ALP was significantly higher in MTA P compared with MTA ( P < 0.05). Conclusions MTA and MTA Plus were noncytotoxic, increased mineralization processes in vitro and induced the expression of osteogenic markers.