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Osteogenic cell response to calcium aluminate‐based cement
Author(s) -
CastroRaucci L. M. S.,
Teixeira L. N.,
Oliveira I. R.,
RaucciNeto W.,
Jacobovitz M.,
Rosa A. L.,
Oliveira P. T.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12682
Subject(s) - bone sialoprotein , mineral trioxide aggregate , osteocalcin , alkaline phosphatase , osteoblast , calvaria , chemistry , viability assay , osteopontin , calcium , in vitro , microbiology and biotechnology , dentistry , biology , medicine , biochemistry , endocrinology , organic chemistry , enzyme
Aim To evaluate the effect of a calcium aluminate‐based cement ( CAC +) on the development of the osteogenic phenotype in vitro . Methodology Rat calvaria‐derived cells were grown on Thermanox ® coverslips for 24 h and then exposed to either samples (4‐h set) of CAC + or mineral trioxide aggregate ( MTA ) placed on Transwell ® inserts for periods of up to 14 days. Nonexposed cultures were used as the controls. The comparisons were made using the nonparametric Kruskal–Wallis test, followed by the Student–Newman–Keuls post hoc test when appropriate. Results The results showed that proximity to MTA or CAC + samples inhibited cell growth, whereas at a distance, viable and proliferative cells adhered to and spread on the Thermanox ® , expressing osteoblast differentiation markers prior to mineralization of the extracellular matrix. Compared with MTA , the osteogenic cell cultures exposed to CAC + exhibited significantly greater cell viability, alkaline phosphatase ( ALP ) activity and expression of runt‐related transcription factor 2, osterix, ALP , bone sialoprotein and osteocalcin ( P < 0.05 for all). For the osteogenic cell cultures exposed to CAC +, the quantification of matrix mineralization was not altered ( P > 0.05). Conclusions CAC + supported the acquisition of the osteogenic cell phenotype in vitro , rendering this novel material a potential alternative to MTA in endodontic procedures. Further in vivo studies are needed to verify if the beneficial in vitro effects of CAC + on osteoblastic cells correspond to an increase and/or acceleration of bone repair in the periapical region.