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Expression of KLF5 in odontoblastic differentiation of dental pulp cells during in vitro odontoblastic induction and in vivo dental repair
Author(s) -
Han N.,
Chen Z.,
Zhang Q.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12672
Subject(s) - dentin sialophosphoprotein , odontoblast , alkaline phosphatase , chemistry , pulp (tooth) , dentinogenesis , pulp capping , cellular differentiation , western blot , dental papilla , downregulation and upregulation , in vitro , microbiology and biotechnology , pathology , biology , medicine , biochemistry , gene , enzyme
Aim To identify whether Krüppel‐like factor 5 ( KLF 5) was involved in odontoblastic differentiation during reparative dentine formation. Methodology Human Dental pulp cells (DPCs) were isolated from healthy human dental pulp tissue and induced for odontoblastic differentiation. Alizarin Red staining, alkaline phosphatase ( ALP ase) activity, quantitative real‐time PCR and Western Blot were performed to evaluate in vitro odontoblastic differentiation. The expression profile of KLF 5 during the in vitro odontoblastic differentiation was determined by quantitative real‐time PCR and Western Blot. Knock‐down of KLF 5 by lentivirus‐mediated sh RNA was performed to determine the function of KLF 5 in odontoblastic differentiation. After direct pulp capping with MTA , the maxillary first molar segments dissected from male Wistar rats were prepared for histology analysis and immunohistochemistry staining. Results Odontoblastic differentiation was confirmed by significantly increased alkaline phosphatase ( ALP ; P = 0.004) activity and upregulated odontoblastic differentiation‐related genes including dentine sialophosphoprotein ( DSPP ; P = 0.004) and dentine matrix protein‐1 ( DMP ‐1; P = <0.001). The expression of KLF 5 was significantly upregulated during odontoblastic differentiation of in vitro cultured DPC s ( P = 0.0002). KLF 5 knock‐down impaired odontoblastic differentiation. After direct pulp capping, dentine bridge‐like calcified tissues were formed under the perforation sites. KLF 5 was expressed in odontoblast‐like cells and DPC s beneath the perforation sites during reparative dentine formation. Conclusions KLF 5 might be involved in the process of odontoblastic differentiation during reparative dentine formation.