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Transdentinal photobiostimulation of stem cells from human exfoliated primary teeth
Author(s) -
Turrioni A. P.,
Basso F. G.,
Montoro L. A.,
Almeida L. F. D.,
Souza Costa C. A.,
Hebling J.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12665
Subject(s) - alkaline phosphatase , molar , viability assay , irradiation , odontoblast , deciduous teeth , andrology , mtt assay , dentistry , chemistry , microbiology and biotechnology , materials science , cell , dentin , biology , medicine , biochemistry , enzyme , physics , nuclear physics
Aim To evaluate the effects of infrared light‐emitting diode ( LED ) irradiation on stem cells from human exfoliated deciduous teeth ( SHED s). Methodology Exfoliated primary teeth were obtained ( n = 3), and SHED s obtained from the teeth were seeded on the pulpal surface of 0.2‐mm‐thick dentine discs produced from permanent molars. The cells were incubated for 24 h by placing the discs in plain Dulbecco's modified Eagle's medium ( DMEM ). The DMEM was then replaced with new culture medium formulated for odontoblast differentiation. After 12 h in the second medium, SHED s were irradiated through the dentine discs using an infrared LED (850 nm) with a power density of 80 mW cm −2 . Energy doses (EDs) delivered to the occlusal surface of the dentine discs were 0 (control), 2 and 4 J cm −2 ( n = 6). Subsequent tests were performed 72 h after irradiation. These tests included cell viability ( MTT ), alkaline phosphatase activity ( ALP ), total protein production ( TP ), scanning electron microscopy ( SEM ), as well as gene expression for ALP , Col I, DSPP and DMP ‐1. Data were analysed using Kruskal–Wallis and Mann–Whitney t ‐tests (α = 0.05). Results Both EDs (2 and 4 J cm −2 ) significantly increased cell viability and ALP activity. For TP , ALP and Col I gene expression, only the 4 J cm −2 group had significantly higher values compared to the control group. Cell morphology was not affected by irradiation. Conclusion Infrared LED irradiation was capable of biostimulating SHED s through a 0.2 mm thickness of dentine, especially at the 4 J cm −2 level.