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Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model
Author(s) -
Waal S. V.,
Almeida J.,
Krom B. P.,
Soet J. J.,
Crielaard W.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12634
Subject(s) - biofilm , sodium hypochlorite , diffusion , antimicrobial , chemistry , microbiology and biotechnology , salt (chemistry) , materials science , bacteria , biology , organic chemistry , genetics , physics , thermodynamics
Aim To describe the application of a newly‐developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied. Methodology In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side‐channel biofilm was selected and treated with phosphate buffered saline, 2% sodium hypochlorite (NaOCl), 17% ethylenediaminetetra‐acetic acid ( EDTA ) or modified salt solution ( MSS ). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min. Results It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of Na OC l into the biofilms was seen after its application. Seventeen‐percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application. Conclusions This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible.