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Powdered coconut water as a storage medium to preserve the viability of periodontal ligament cells: a laboratory study
Author(s) -
Moura C. C. G.,
Soares P. B. F.,
Reis M. V. P.,
Dechichi P.,
Salgueiro C. C. M.,
Sobral M. H. N. R.,
Zanetta Barbosa D.,
Soares C. J.
Publication year - 2017
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12603
Subject(s) - osmole , periodontal fiber , trypan blue , zoology , chemistry , significant difference , negative control , positive control , tukey's range test , food science , dentistry , biology , biochemistry , in vitro , medicine , horticulture , traditional medicine
Aim To investigate the ability of newly developed powdered coconut water formulas ( ACP ) with different osmolarities to maintain the viability of periodontal ligament ( PDL ) cells over time compared with other solutions. Methodology Dogs teeth were extracted and stored for two periods, 3 h or 24 h, in the following media: long‐shelf life CW ( CW ), pH ‐adjusted long‐shelf life CW ( pH ‐ CW ) and powdered CW that was pH and osmolality adjusted ( ACP ‐404‐I, 250 mOsm kg −1 H 2 O; pH 7.0; ACP ‐404‐ II , 372 mOsm kg −1 H 2 O; pH 7.0; ACP ‐404‐ III , 300 mOsm kg −1 H 2 O; pH 7.4). The positive control group (Pc) corresponded to immediate measurement after tooth extraction, and two negative controls (Nc) corresponded to 3 h and 24 h of dry time. PDL cells were extracted, and cell viability analysed by Trypan blue exclusion. Data were analysed statistically using two‐way anova followed by the Tukey test and one‐way anova followed by the Dunnett test ( P < 0.05). Results At 3 h and 24 h, ACP ‐404‐I had a performance similar to those of ACP ‐404‐ II and pH ‐ CW , with significantly higher ( P = 0.004) percentages of viable cells than ACP ‐404‐ III and CW . The positive control group had a significantly higher ( P = 0.002) percentage of viable cells than the negative control groups, CW and ACP ‐404‐ III , irrespective of the period evaluated. Conclusion Powdered coconut water formulas, ACP ‐404‐I and ACP ‐404‐ II , preserved viability for up to 24 h.