Premium
DNA double‐strand breaks caused by new and contemporary endodontic sealers
Author(s) -
Eldeniz A. U.,
Shehata M.,
Högg C.,
Reichl F. X.
Publication year - 2016
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12577
Subject(s) - cytotoxicity , microbiology and biotechnology , genotoxicity , telomerase , chemistry , dentistry , in vitro , biology , medicine , biochemistry , gene , organic chemistry , toxicity
Aim To investigate the cytotoxicity and genotoxicity of a new silicate‐based BioRoot RCS ® sealer in comparison with contemporary sealers. Methodology A periodontal ligament cell line using lentiviral gene transfer of human telomerase reverse transcriptase ( hTERT ) was used and exposed to subtoxic concentrations of 24‐h eluates from two epoxy resin‐based ( AH Plus Jet ® and Acroseal ® ), four various methacrylate‐based endodontic sealers (Endo REZ ® , RealSeal ® , RealSeal SE ® and Hybrid Root SEAL ® ) and three silicate‐based sealers (BioRoot RCS ® , iR oot SP ® and MTA Fillapex ® ). The XTT ‐based cell viability assay was used for cytotoxicity screening of materials. The γ‐H2 AX assay was used for genotoxicity screening. In the γ‐H2 AX immunofluorescence assay, PDL ‐ hTERT cells were exposed to eluates of the substances for 6 h and DNA double‐strand breaks ( DSB ) were detected microscopically. Induced foci represented DSB s, which can induce ATM ‐dependent phosphorylation of the histone H2 AX . The statistical significance of the differences between the experimental groups was compared using the Student's t ‐test ( P < 0.05). Results The cytotoxicity of the 24‐h eluates could be ranked in the following order: Hybrid Root SEAL ® >RealSeal ® >Acroseal ® >RealSeal SE ® ≥ AH Plus Jet ® > Endo REZ ® > MTA Fillapex ® > iR oot SP ® >BioRoot RCS ® . In negative controls (cells which received medium only) 4.08 ± 0.53 DSB foci (mean ± SEM ) whilst in positive controls 10.76 ± 4.05 DSB foci/cell were found. BioRoot RCS ® and RealSeal SE ® exhibited significant differences in foci formation at 1/3 EC 50 compared with their 1/10 EC 50 concentration (P < 0.05). Both concentrations (1/10 and 1/3 of EC 50) of AH Plus Jet ® , Acroseal ® , RealSeal ® and MTA Fillapex ® sealers were not significantly different when compared with the medium control ( P < 0.05). Conclusions New BioRoot RCS ® was not toxic whilst Hybrid Root SEAL ® demonstrated more toxicity and DNA double‐strand breaks when compared with other resin‐ and silicate‐based root canal sealers.