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EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells
Author(s) -
Galler K. M.,
Widbiller M.,
Buchalla W.,
Eidt A.,
Hiller K. A.,
Hoffer P. C.,
Schmalz G.
Publication year - 2016
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12492
Subject(s) - dental pulp stem cells , chemistry , viability assay , dentistry , pulp (tooth) , molar , stem cell , conditioning , cell adhesion , root canal , microbiology and biotechnology , cell , biology , medicine , biochemistry , statistics , mathematics
Aim To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. Methodology Dentine discs prepared from extracted human molars were pre‐treated with EDTA (10%), NaOCl (5.25%) or H 2 O. Migration of dental pulp stem cells towards pre‐treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evaluated indirectly by measuring cell viability. Expression of mineralization‐associated genes (COL1A1, ALP, BSP, DSPP, RUNX2) in cells cultured on pre‐treated dentine for 7 days was determined by RT‐qPCR. Nonparametric statistical analysis was performed for cell migration and cell viability data to compare different groups and time‐points (Mann–Whitney U ‐test, α = 0.05). Results Treatment of dentine with H 2 O or EDTA allowed for cell attachment, which was prohibited by NaOCl with statistical significance ( P = 0.000). Furthermore, EDTA conditioning induced cell migration towards dentine. The expression of mineralization‐associated genes was increased in dental pulp cells cultured on dentine after EDTA conditioning compared to H 2 O‐pre‐treated dentine discs. Conclusions EDTA conditioning of dentine promoted the adhesion, migration and differentiation of dental pulp stem cells towards or onto dentine. A pre‐treatment with EDTA as the final step of an irrigation protocol for regenerative endodontic procedures has the potential to act favourably on new tissue formation within the root canal.