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Stem cell‐based pulp tissue engineering: variables enrolled in translation from the bench to the bedside, a systematic review of literature
Author(s) -
Conde M. C. M.,
Chisini L. A.,
Demarco F. F.,
Nör J. E.,
Casagrande L.,
Tarquinio S. B. C.
Publication year - 2016
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12489
Subject(s) - pulp (tooth) , stem cell , regeneration (biology) , tissue engineering , biomedical engineering , dental pulp stem cells , scaffold , dentistry , chemistry , medicine , microbiology and biotechnology , biology
Stem cell‐based therapy ( SC ‐ BT ) is emerging as an alternative for endodontic therapies. The interaction between stem cells and scaffolds plays a crucial role in the generation of a ‘friendly cell’ microenvironment. The aim of this systematic review was to explore techniques applied to regenerate the pulp–dentine complex tissue using SC ‐ BT . An electronic search into the SciVerse Scopus ( SS ), ISI Web Science ( IWS ) and Entrez PubMed ( EP ) using specific keywords was performed. Specific inclusion and exclusion criteria were predetermined. The search yielded papers, out of which full‐text papers were included in the final analyses. Data extraction pooled the results in four main topics: (a) influence of the chemical properties of the scaffolds over cell behaviour; (b) influence of the physical characteristics of scaffolds over cell behaviour; (c) strategies applied to improve the stem cell/scaffold interface; and (d) influence of cue microenvironment on stem cell differentiation towards odontoblast‐like cells and pulp‐like tissue formation. The relationship between the scaffolds, the environment and the growth factors released from dentine are critical for de novo pulp tissue regeneration. The preconditioning of dentine walls with ethylenediaminetetraacetic acid ( EDTA ) was imperative for successful pulp–dentine complex regeneration. An analyses of the grouped results revealed that pulp regeneration was an attainable goal.