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Efficacy of low concentrations of sodium hypochlorite and low‐powered Er,Cr: YSGG laser activated irrigation against an Enterococcus faecalis biofilm
Author(s) -
Christo J. E.,
Zilm P. S.,
Sullivan T.,
Cathro P. R.
Publication year - 2016
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12447
Subject(s) - enterococcus faecalis , sodium hypochlorite , saline , chemistry , colony forming unit , dentistry , nuclear chemistry , materials science , bacteria , medicine , staphylococcus aureus , biology , organic chemistry , genetics , endocrinology
Aim To establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without Er,Cr: YSGG laser activation on Enterococcus faecalis biofilms in extracted teeth. Methodology The root canals of 96 decoronated single‐rooted extracted human teeth were prepared to a size 40, 0.06 taper 1 mm beyond the apex. They were mounted within a flow cell, which was sterilized before pumping a nutrient media through the root canals. The flow cell was inoculated with E. faecalis ( ATCC 700802) and cultivated for 4 weeks. The root‐ends were sealed, and the roots were then subjected to one of six treatment groups: group 1: syringe irrigation ( SI ) with saline (control) using a 27 ‐gauge Monoject needle 1 mm from the apex for 2 min; group 2: as for group 1 but with 1% Na OC l; group 3: as for group 1 but with 4% Na OC l; group 4: 0.5% Na OC l irrigation for 15 s followed by laser‐activated irrigation ( LAI ) with four 15‐s cycles replenishing the irrigant between cycles; group 5: as for group 4 but with 1% Na OC l as the irrigant; group 6: as for group 4 but with 4% Na OC l as the irrigant. Following treatment, teeth were crushed and viable bacteria were quantitated by serial dilution and plating. The colony‐forming unit values were compared between groups using one‐way anova and Tukey‐adjusted post hoc tests. A two‐tailed P value of <0.05 was considered statistically significant. Results The mean number of cells recovered from the 1% Na OC l SI group was significantly higher than that from the 4% Na OC l LAI group ( P = 0.02). Conclusion Within the limitations of this laboratory study, low‐powered (0.5 W) Er,Cr: YSGG laser activation did not improve the antibacterial effect of low concentrations of sodium hypochlorite.