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Combinations of bacterial species associated with symptomatic endodontic infections in a Chinese population
Author(s) -
Qi Z.,
Cao H.,
Jiang H.,
Zhao J.,
Tang Z.
Publication year - 2016
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12428
Subject(s) - tannerella forsythia , porphyromonas gingivalis , forsythia , microbiology and biotechnology , prevotella intermedia , population , fusobacterium nucleatum , 16s ribosomal rna , biology , treponema denticola , bacteria , medicine , pathology , traditional chinese medicine , honeysuckle , alternative medicine , environmental health , genetics
Abstract Aim To use microarrays to detect 11 selected bacteria in infected root canals, revealing bacterial combinations that are associated with clinical symptoms and signs of primary endodontic infections in a Chinese population. Methodology DNA was extracted from 90 samples collected from the root canals of teeth with primary endodontic infections in a Chinese population, and the 16S rRNA gene was amplified by polymerase chain reaction ( PCR ). The PCR products were hybridized to microarrays containing specific oligonucleotide probes targeting 11 species, and the arrays were screened with a confocal laser scanner. Pearson's chi‐squared test and cluster analysis were performed to investigate the associations between the bacterial combinations and clinical symptoms and signs using SAS 8.02. Results Seventy‐seven samples (86%) yielded at least one of the 11 target species. Parvimonas micra (56%), Porphyromonas endodontalis (51%), Tannerella forsythia (48%), Prevotella intermedia (44%) and Porphyromonas gingivalis (37%) were the most prevalent taxa and were often concomitant. The following positive associations were found between the bacterial combinations and clinical features: P. endodontalis and T. forsythia with abscess; P. gingivalis and P. micra with sinus tract; P. gingivalis and P. endodontalis or P. micra and P. endodontalis with abscess and sinus tract; and the combination of P. endodontalis , P. micra , T. forsythia and P. gingivalis with sinus tract ( P < 0.05). Conclusions Various combinations of P. micra , P. endodontalis , T. forsythia and P. gingivalis may contribute to abscesses or sinus tracts of endodontic origin with bacterial synergism in a Chinese population.