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Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms
Author(s) -
AriasMoliz M. T.,
OrdinolaZapata R.,
Baca P.,
RuizLinares M.,
García García E.,
Hungaro Duarte M. A.,
Monteiro Bramante C.,
FerrerLuque C. M.
Publication year - 2015
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12424
Subject(s) - peracetic acid , enterococcus faecalis , sodium hypochlorite , chlorhexidine , distilled water , biofilm , antimicrobial , chemistry , microbiology and biotechnology , dentistry , bacteria , medicine , biology , biochemistry , chromatography , escherichia coli , genetics , organic chemistry , gene , hydrogen peroxide
Aim To evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (Na OC l) and associated with 9% HEBP (Na OC l/ HEBP ), 2% peracetic acid ( PAA ) and 2% chlorhexidine ( CHX ), on the viability of Enterococcus faecalis biofilms attached to dentine. Methodology Biofilms of E. faecalis were grown on the surface of dentine blocks for 5 days and then exposed to the irrigating solutions for 3 min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences ( P < 0.05). Results Na OC l and the Na OC l/ HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA ( P < 0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in Na OC l, Na OC l/ HEBP and PAA solutions in comparison with the CHX and control groups. Conclusions Na OC l alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing E. faecalis biofilms.