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Relationships between the antibacterial activity of sodium hypochlorite and treatment time and biofilm age in early Enterococcus faecalis biofilms
Author(s) -
Chau N. P. T.,
Chung N. H.,
Jeon J. G.
Publication year - 2015
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12376
Subject(s) - enterococcus faecalis , biofilm , antibacterial activity , sodium hypochlorite , microbiology and biotechnology , chemistry , colony forming unit , enterococcus , bacteria , biology , staphylococcus aureus , antibiotics , organic chemistry , genetics
Aim To determine the relationships between the antibacterial activity of Na OC l and treatment time and biofilm age in early Enterococcus faecalis biofilms using a linear fitting procedure. Methodology Enterococcus faecalis biofilms were formed on hydroxyapatite discs. To investigate the relationship between the antibacterial activity of Na OC l and biofilm age, 22‐, 46‐, 70‐ and 94‐h‐old biofilms were exposed to Na OC l (0–3%) for 5 min. To investigate the relationship between the antibacterial activity of Na OC l and treatment time, 70‐h‐old biofilms were exposed to Na OC l (0–3%) for 1, 3, 5 and 7 min. After treatment, colony‐forming units ( CFU s) were counted. To determine the relationships between these variables, linear fitting was performed. Results The change in the minimum biofilm eradication concentration ( MBEC ) of Na OC l followed a linear pattern of biofilm age ( R = 0.941, R 2 = 0.886) or treatment time dependence ( R = −0.948, R 2 = 0.898). Below the MBEC , the fitting lines for bacterial CFU count versus Na OC l concentration ( R ≤ −0.973, R 2 ≥ 0.948) in the 22‐, 46‐, 70‐ and 94‐h‐old biofilms implied that the antibacterial activity of Na OC l decreased as the biofilm age increased. The fitting lines for bacterial CFU count versus Na OC l concentration ( R ≤ −0.970, R 2 ≥ 0.942) in the 1‐, 3‐, 5‐ and 7‐min treatments implied that the antibacterial activity of Na OC l increased with treatment time. Conclusions These results suggest that the antibacterial activity of Na OC l against early E. faecalis biofilms in root canals may follow a linear pattern depending on biofilm age or treatment time.