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Immunoexpression of tryptase‐positive mast cells in periapical granulomas and radicular cysts
Author(s) -
Costa Neto H.,
Andrade A. L. D. L.,
GordónNúñez M. A.,
Freitas R. de A.,
Galvão H. C.
Publication year - 2015
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12366
Subject(s) - tryptase , pathology , immunohistochemistry , radicular cyst , immunostaining , mast cell , mast (botany) , medicine , lesion , chemistry , immunology , odontogenic
Aim To evaluate and compare the immunoexpression of tryptase in samples of periapical granulomas ( PG s) and radicular cysts ( RC s) correlating it with the type of lesion, localization, intensity of the inflammatory infiltrate and thickness of the cystic epithelial lining, in order to gain insight into the phlogistic role of these cells in the lesions studied. Methodology Twenty‐five PG s and twenty‐five RC s obtained from human teeth without endodontic treatment were submitted to morphological and immunohistochemical analysis using anti‐tryptase antibody. Mast cells were identified and counted in three regions: intra‐epithelial, central/superficial and deep portions. The data were analysed using the M ann– W hitney U ‐test ( P < 0.05). Results In comparison with RC s, PG s exhibited higher immunoexpression of tryptase‐positive mast cells located in both central/superficial and deep regions ( P < 0.001 and P < 0.001, respectively). When considering the total number of mast cells and disregarding the location, the number of tryptase‐positive mast cells increased gradually from RC s to PG s ( P < 0.001). Lesions with inflammatory infiltrate grade III had greater number of tryptase‐positive mast cells located in both central/superficial and deep regions than lesions with inflammatory infiltrates grade II ( P = 0.045 and P = 0.025). When the location was ignored, the lesions with inflammatory infiltrate grade III also exhibited higher immunostaining of tryptase‐positive mast cells ( P = 0.01). Conclusions Tryptase‐positive mast cells were present in chronic periapical lesions in a larger number in periapical granulomas than in radicular cysts, in both central/superficial and deep regions.