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Expression and immunohistochemical localization of leptin receptor in human periapical granuloma
Author(s) -
MartínGonzález J.,
CarmonaFernández A.,
PérezPérez A.,
SánchezJiménez F.,
SánchezMargalet V.,
SeguraEgea J. J.
Publication year - 2015
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12356
Subject(s) - immunohistochemistry , leptin , leptin receptor , western blot , monoclonal antibody , pathology , biology , antibody , medicine , immunology , gene , obesity , genetics
Abstract Aim To investigate the expression and immunohistochemical localization of leptin receptor ( LEPR ) in human periapical granulomas. Methodology Periapical inflammatory lesions were obtained from extracted human teeth and teeth which underwent periapical surgery. After their histopathological categorization as periapical granulomas ( n = 20), they were examined by immunohistochemistry using human LEPR monoclonal antibodies. LEPR mRNA expression was also determined by quantitative real‐time PCR ( qRT ‐PCR), and the amount of LEPR protein was analysed by immunoblot. Results All granuloma samples expressed LEPR. Amongst inflammatory cells, only macrophages showed expression of LEPR. Western blot analysis revealed the presence in the samples of a protein with apparent molecular weight of ~120 kDa, corresponding to the estimated molecular weight of LEPR. The qRT ‐PCR analysis demonstrated the expression of LEPR mRNA , corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR mRNA . Conclusions Human periapical granulomas express LEPR . In periapical granulomas, only macrophages showed expression of LEPR . This finding suggests that leptin can play a role in inflammatory and immune periapical responses.