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Catechins inhibit vascular endothelial growth factor production and cyclooxygenase‐2 expression in human dental pulp cells
Author(s) -
Nakanishi T.,
Mukai K.,
Hosokawa Y.,
Takegawa D.,
Matsuo T.
Publication year - 2015
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12312
Subject(s) - vascular endothelial growth factor , lipopolysaccharide , cyclooxygenase , pulp (tooth) , tumor necrosis factor alpha , vascular endothelial growth factor a , interleukin 8 , inflammation , chemistry , cancer research , pharmacology , medicine , immunology , vegf receptors , biochemistry , enzyme , pathology
Aim To investigate the effect of catechins on vascular endothelial growth factor ( VEGF ) production and cyclooxygenase‐2 ( COX ‐2) expression in human dental pulp cells ( HDPC ) stimulated with bacteria‐derived factors or pro‐inflammatory cytokines. Methodology Morphologically fibroblastic cells established from explant cultures of healthy human dental pulp tissues were used as HDPC . HDPC pre‐treated with catechins, epigallocatechin‐3‐gallate ( EGCG ) or epicatechin gallate ( ECG ), were exposed to lipopolysaccharide ( LPS ), peptidoglycan ( PG ), interlukin‐1β ( IL ‐1β) or tumour necrosis factor‐α ( TNF ‐α). VEGF production was examined by enzyme‐linked immunosorbent assay, and COX ‐2 expression was assessed by immunoblot. Results EGCG and ECG significantly reduced LPS‐ or PG‐mediated VEGF production in the HDPC in a dose‐dependent manner. EGCG also prevented IL‐1β‐mediated VEGF production. Although TNF‐α did not enhance VEGF production in the dental pulp cells, treatment of 20 μg mL −1 of EGCG decreased the level of VEGF. In addition, the catechins attenuated COX‐2 expression induced by LPS and IL‐1β. Conclusions The up‐regulated VEGF and COX ‐2 expressions in the HDPC stimulated with these bacteria‐derived factors or IL ‐1β were diminished by the treatment of EGCG and ECG . These findings suggest that the catechins may be beneficial as an anti‐inflammatory tool of the treatment for pulpal inflammation.