In vitro biocompatibility of a dentine substitute cement on human MG 63 osteoblasts cells: B iodentine™ versus MTA ®

Aim To compare the in vitro biocompatibility of Biodentine™ and White ProRoot ® mineral trioxide aggregate ( MTA ® ) with MG 63 osteoblast‐like cells and to characterize the cement surface. Methodology A direct contact model for MG 63 osteoblast‐like cells with cements was used for 1, 3 and 5 days. Four end‐points were investigated: (i) cement surface characterization by atomic force microscopy ( AFM ), (ii) cell viability by MTT assay, (iii) protein amount quantification by B radford assay and (iv) cell morphology by SEM . Statistical analyses were performed by analysis of variance ( anova ) with a repetition test method. Results The roughness of the cements was comparable as revealed by AFM analysis. The MTT test for B iodentine™ was similar to that of MTA ® . B iodentine™ and MTA ® induced a similar but slight decrease in metabolic activity. The amount of total protein was significantly enhanced at day three ( P  < 0.05) but slightly decreased at day five for both tested samples. B iodentine™ was tolerated as well as MTA ® in all cytotoxicity assays. SEM observations showed improvement of cell attachment and proliferation on both material surfaces following the three incubation periods. Conclusion The biocompatibility of B iodentine™ to bone cells was comparable to MTA ® .