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In vitro biocompatibility of a dentine substitute cement on human MG 63 osteoblasts cells: B iodentine™ versus MTA ®
Author(s) -
Attik G. N.,
Villat C.,
Hallay F.,
PradellePlasse N.,
Bonnet H.,
Moreau K.,
Colon P.,
Grosgogeat B.
Publication year - 2014
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12261
Subject(s) - mineral trioxide aggregate , biocompatibility , viability assay , osteoblast , mtt assay , materials science , atomic force microscopy , cement , cytotoxicity , in vitro , dentistry , nuclear chemistry , chemistry , nanotechnology , biochemistry , medicine , composite material , metallurgy
Aim To compare the in vitro biocompatibility of Biodentine™ and White ProRoot ® mineral trioxide aggregate ( MTA ® ) with MG 63 osteoblast‐like cells and to characterize the cement surface. Methodology A direct contact model for MG 63 osteoblast‐like cells with cements was used for 1, 3 and 5 days. Four end‐points were investigated: (i) cement surface characterization by atomic force microscopy ( AFM ), (ii) cell viability by MTT assay, (iii) protein amount quantification by B radford assay and (iv) cell morphology by SEM . Statistical analyses were performed by analysis of variance ( anova ) with a repetition test method. Results The roughness of the cements was comparable as revealed by AFM analysis. The MTT test for B iodentine™ was similar to that of MTA ® . B iodentine™ and MTA ® induced a similar but slight decrease in metabolic activity. The amount of total protein was significantly enhanced at day three ( P < 0.05) but slightly decreased at day five for both tested samples. B iodentine™ was tolerated as well as MTA ® in all cytotoxicity assays. SEM observations showed improvement of cell attachment and proliferation on both material surfaces following the three incubation periods. Conclusion The biocompatibility of B iodentine™ to bone cells was comparable to MTA ® .