Premium
Kinetics of glycogen synthase kinase ( GSK )3β and phosphorylated GSK 3β (Ser 9) expression in experimentally induced periapical lesions
Author(s) -
Sun Z.,
Wang L.,
Peng B.
Publication year - 2014
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12258
Subject(s) - western blot , gsk 3 , immunohistochemistry , immunofluorescence , gsk3b , pathology , lesion , chemistry , medicine , microbiology and biotechnology , kinase , biology , antibody , immunology , biochemistry , gene
Aim To investigate the kinetics of GSK 3β and p‐ GSK 3β (Ser 9) expression in experimentally induced rat periapical lesions and to explore their possible functions in the pathogenesis of periapical lesions. Methodology Periapical lesions were established in Wistar rats by occlusal pulp exposure in mandibular first molar teeth. The animals were killed on days 0, 7, 14, 21 and 28. Micro‐computed tomographic, histological and enzyme histochemical analyses were performed to detect the progression of periapical lesions. Immunohistochemistry, double‐dye immunofluorescence and Western blot were performed to determine the expression of GSK 3β and p‐ GSK 3β (Ser 9) in periapical tissues. Results From day 0 to day 28, the lesion volume and area gradually expanded, and the GSK 3β‐positive cells gradually ascended. A few p‐ GSK 3β (Ser 9)‐positive cells and osteoclasts appeared on day 7 and then climaxed on day 14. The numbers then simultaneously decreased from day 21 to day 28. Western blot analysis revealed that p‐ GSK 3β (Ser 9) and GSK 3β proteins were expressed at all time‐points. The positive cells and protein expression ratio of p‐ GSK 3β (Ser 9) against GSK 3β increased from day 0 to day 14 and then decreased from day 14 to day 28. Finally, double‐dye immunofluorescence assay revealed that p‐ GSK 3β (Ser 9)‐positive and RANKL ‐positive cells were co‐localized around periapical lesions on days 14 and 28. Conclusions GSK 3β and p‐ GSK 3β (Ser 9) can be observed and may be involved in alveolar bone resorption and inflammatory response in periapical lesions, as well as associated with periapical lesion pathogenesis.