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Pro‐inflammatory cytokine release and cell growth inhibition in primary human oral cells after exposure to endodontic sealer
Author(s) -
Diomede F.,
Caputi S.,
Merciaro I.,
Frisone S.,
D'Arcangelo C.,
Piattelli A.,
Trubiani O.
Publication year - 2014
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12230
Subject(s) - periodontal ligament stem cells , periodontal fiber , dental pulp stem cells , proinflammatory cytokine , cytokine , tumor necrosis factor alpha , interleukin 8 , stem cell , andrology , cell growth , ex vivo , pulp (tooth) , interleukin 10 , interleukin , chemistry , microbiology and biotechnology , dentistry , immunology , in vitro , medicine , biology , inflammation , biochemistry , alkaline phosphatase , enzyme
Abstract Aim To assay the toxicity of the single‐methacrylate‐based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro‐inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells ( hDPSC s), human periodontal ligament stem cells ( hPDLSC s), human gingival fibroblasts ( hGF s) and human osteoblasts ( hOST s). Methodology Dental pulp and periodontal ligament stem cells, osteoblasts and fibroblasts were derived from five young donors. After in vitro isolation, hDPSC s, hPDLSC s, hGF s and hOST s were seeded to resin‐based sealers for 24, 48, 72 h up to 1 week. The morphological features and the cell growth and the release of pro‐inflammatory interleukin (IL)6, IL8, IL12 and tumour necrosis factor (TNF) α were analysed. Differences in cell growth and in interleukin secretion were analysed for statistical significance with two‐way anova tests for multiple comparisons. Results Exposure to endodontic sealer based on UDMA resulted in a 50% decrease in survival oral cells at 24 h of incubation. No evident morphological changes were present in cell cultures examined. After 48 h, 72 h and 1‐week culture time, a progressive cell growth was evident. A significant up‐regulation of IL 6, IL 8, IL 12 and TNF α cytokines in cells in contact with the dental sealer compared to the control was observed. Conclusion In vitro , EndoRez interacted with primary human hDPSC s, hPDLSC s, hGF s and hOST s causing damage to biological system evidenced through cell growth inhibition and up‐regulation of IL6, IL8, IL12 and TNFα proinflammatory mediators.