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The synergistic effects of fibroblast growth factor‐2 and mineral trioxide aggregate on an osteogenic accelerator in vitro
Author(s) -
Liu C.H.,
Huang T.H.,
Hung C.J.,
Lai W.Y.,
Kao C.T.,
Shie M.Y.
Publication year - 2014
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12227
Subject(s) - mineral trioxide aggregate , transfection , fibroblast growth factor , fibroblast , materials science , in vitro , microbiology and biotechnology , osteocalcin , small interfering rna , chemistry , alkaline phosphatase , cell culture , biology , dentistry , receptor , biochemistry , medicine , enzyme , genetics
Aim To examine the effects of mineral trioxide aggregate ( MTA )/fibroblast growth factor‐2 ( FGF ‐2) on material properties and in vitro human dental pulp cell (h DPC s) behaviour. Methodology The setting time and diametral tensile strength ( DTS ) of MTA and MTA / FGF ‐2 were measured. The structure of specimens before and after soaking in DMEM was examined under a scanning electron microscope. Alamar B lue was used for evaluating h DPC s proliferation. An enzyme‐linked immunosorbent assay was employed to determine ALP and osteocalcin ( OC ) expression in h DPC s cultured on cements. The effect of small interfering RNA (si RNA ) transfection targeting fibroblast growth factor receptor ( FGFR ) was also evaluated. One‐way analysis of variance was used to evaluate the significance of the differences between the mean values. Results Setting time and DTS data were not found to be significant ( P > 0.05) between MTA with and without FGF ‐2. Cell proliferation and differentiation increased significantly ( P < 0.05) with FGF ‐2 mixed MTA . After si RNA transfection with FGFR , the proliferation and differentiation behaviour of the hDPC s appreciably decreased when cultured on an MTA / FGF ‐2 composite. In contrast, no significant amounts ( P > 0.05) of ALP and OC were secreted by hDPC s seeded on MTA . Conclusions Mineral trioxide aggregate with FGF ‐2 content enhanced the higher expression of hDPC s proliferation and osteogenic differentiation as compared to pure MTA cement.