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Effects of bacterial products on the activity of odontoblast‐like cells and their formation of type 1 collagen
Author(s) -
Fransson H.,
Petersson K.,
Davies J. R.
Publication year - 2014
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12160
Subject(s) - enterococcus faecalis , microbiology and biotechnology , odontoblast , bacteria , chemistry , mtt assay , lesion , extracellular , biofilm , lipoteichoic acid , lipopolysaccharide , in vitro , biology , biochemistry , pathology , dentin , staphylococcus aureus , immunology , medicine , genetics
Aim To study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast‐like cells and their ability to produce the major organic component of dentine, collagen 1. Methodology MDPC ‐23 cells were exposed to supernatants from biofilm cultures of strains isolated from the deepest part of a carious lesion as well as from a clinical isolate of E nterococcus faecalis . Lipoteichoic acid ( LTA ) and lipopolysaccharide ( LPS ) were used for comparison. Cell activity was assessed using an methyl‐thiazolyl‐diphenyl tetrazolium bromide ( MTT ) assay, and collagen 1 levels were determined by ELISA . Results The lesion microflora was dominated by L actobacillus spp . Neither extracellular products from the isolates nor LPS affected the activity of the MDPC ‐23 cells, whereas extracellular products from E. faecalis and LTA significantly reduced total cell activity ( P < 0.01). Enterococcus faecalis had an inhibitory effect upon collagen 1 production by the cells, whereas no such effect or even a slight stimulatory effect was seen for the isolates from the deep carious lesion. Conclusions These studies indicate that culture supernatants from E. faecalis reduced the metabolic activity of odontoblast‐like cells as shown using the MTT assay. No effect was seen for supernatants from biofilms of bacteria recovered from a deep carious lesion. Different bacteria varied in their effects upon collagen 1 production suggesting that the nature of the bacterial species in a carious lesion may have a direct influence upon the ability of the odontoblasts to produce tertiary dentine.