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In vitro comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer
Author(s) -
Güven E. P.,
Taşlı P. N.,
Yalvac M. E.,
Sofiev N.,
Kayahan M. B.,
Sahin F.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12115
Subject(s) - mineral trioxide aggregate , bioceramic , dentin sialophosphoprotein , von kossa stain , root canal , alkaline phosphatase , microbiology and biotechnology , staining , viability assay , biology , in vitro , dentistry , andrology , materials science , chemistry , biochemistry , medicine , nanotechnology , genetics , enzyme
Aim To compare the effect of mineral trioxide aggregate (MTA) and iR oot SP, a bioceramic root canal sealer, on the cell viability, hard tissue deposition capacity and odontogenic differentiation of human tooth germ stem cells ( hTGSC s). Methodology The dental materials MTA, iR oot SP and Dycal were packed into Teflon rings and placed on transwell inserts for toxicity evaluations by the MTS assay on days 3 and 7. Dycal was used as a positive control for the cell viability assay. Teflon rings were cocultured with hTGSC s, followed by the induction of odontogenic differentiation. The odontogenic differentiation of hTGSC s and biomineralization ability of the materials were evaluated by analysing the mRNA expression levels of dentine sialophosphoprotein (DSPP) and collagen type 1A (COL1A) by real‐time polymerase chain reaction expression analysis, measurement of alkaline phosphatase (ALP) activity and visualization of calcium deposits by von Kossa staining. Results MTA and iR oot SP exhibited no cytotoxicity, but Dycal caused cytotoxicity ( P < 0.05) of almost all of the cells after 7 days. MTA significantly stimulated ( P < 0.05) the odontogenic differentiation of hTGSC s compared with iR oot SP. MTA and iR oot SP increased ( P < 0.05) the mRNA levels of COL1A and DSPP mRNA compared with noninduced hTGSC s, which served as a negative control (NC). iR oot SP, however, significantly decreased ( P < 0.05) COL1A and DSPP mRNA expression levels compared with the PC. Conclusion MTA and iR oot SP induced hTGSC differentiation into odontoblast‐like cells, but MTA might provide more inductive potential and hard tissue deposition compared with iR oot SP.