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Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet‐rich plasma and dental pulp cells
Author(s) -
Zhu W.,
Zhu X.,
Huang G. T.J.,
Cheung G. S. P.,
Dissanayaka W. L.,
Zhang C.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12087
Subject(s) - dentistry , pulp (tooth) , root canal , periodontitis , medicine , platelet rich plasma , mineral trioxide aggregate , pulpitis , dentin , pulp capping , platelet
Aim To investigate the new tissues growing into the pulp space of immature dog teeth that were infected, disinfected and filled with blood clot ( BC ), dental pulp cells ( DPC s), platelet‐rich plasma ( PRP ) or a combination of DPC s and PRP in immature dog teeth with apical periodontitis. Methodology Fifty‐six immature roots from mandibular premolars of four beagles were divided into four experimental groups ( n = 40) and two control groups. After the induction of apical periodontitis, the root canals of experimental groups were disinfected with Na OC l irrigation and a tri‐antibiotic paste medication. The canals were then filled with different materials according to the experimental group: BC group, DPC s group, PRP group or DPC s + PRP group. Access cavities were sealed with MTA and composite. Radiographs were taken after 90 days, and the jaws including the teeth were processed for histologic analysis. The data were statistically analysed using chi‐square evaluation and Student's t ‐test. Results Radiographic analyses demonstrated no significant difference between experimental groups in periradicular bone healing ( P > 0.05), whilst those groups that used DPC s produced a significantly greater root thickening ( P < 0.01). The histologic evaluation showed that the groups with PRP formed more tissues in the canals ( P = 0.01). The groups with DPC s had substantially more mineralized tissue formation in the canal than those without DPC s, especially in the apical third. In DPC s + PRP group, bone‐like tissue grew into the canal space from the periapical tissue. Conclusions A combination of DPC s + PRP increased vital tissue regeneration within the root canals of immature teeth associated with apical periodontitis.