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Bioactivity evaluation of three calcium silicate‐based endodontic materials
Author(s) -
Han L.,
Okiji T.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12062
Subject(s) - materials science , acicular , nuclear chemistry , root canal , dentinal tubule , distilled water , significant difference , calcium , precipitation , scanning electron microscope , dentistry , chemistry , metallurgy , composite material , chromatography , microstructure , medicine , statistics , mathematics , physics , meteorology
Aim To compare white P ro R oot MTA ( WMTA ), E ndo S equence BC sealer ( BC sealer) and B iodentine with regard to their ability to produce apatites and cause Ca and Si incorporation in adjacent human root canal dentine after immersion in phosphate‐buffered saline ( PBS ). Methodology Root sections of human single‐rooted teeth were filled with one of the materials and immersed in PBS for 1, 7, 30 or 90 days ( n = 5 each). Morphology and elemental composition of surface precipitates and interfacial dentine were analysed using a wavelength‐dispersive X‐ray spectroscopy electron probe microanalyser with image observation function. Ca ‐ and Si ‐incorporation depths in the interfacial dentine were measured. In addition, the amount of Ca ions released from the test materials was measured by EDTA titration. Results All materials produced surface precipitates of acicular or lath‐like morphology with Ca / P ratio of 1.6 : 2.0. Within dentinal tubules, the three materials formed tag‐like structures that were frequently composed of Ca ‐ and P ‐rich and Si ‐poor materials, suggesting intratubular precipitation. Ca ‐ and Si ‐incorporation depths were in the order of B iodentine > WMTA > BC sealer, with a significant difference between BC sealer and the others at several time‐points ( P < 0.05, anova and Tukey's honestly significant difference test). The concentration of released Ca ions was in the order of B iodentine > WMTA > BC sealer with significant differences between the materials ( P < 0.05). Conclusions Compared with B iodentine and WMTA , BC sealer showed less Ca ion release and did not show Ca and Si incorporation as deeply in human root canal dentine when immersed in PBS for up to 90 days.