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Antibiofilm activity, pH and solubility of endodontic sealers
Author(s) -
FariaJúnior N. B.,
TanomaruFilho M.,
Berbert F. L. C. V.,
GuerreiroTanomaru J. M.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12055
Subject(s) - solubility , distilled water , enterococcus faecalis , biofilm , chemistry , dentistry , root canal , materials science , chromatography , medicine , bacteria , biology , biochemistry , genetics , organic chemistry , escherichia coli , gene
Aim To evaluate antibiofilm activity against Enterococcus faecalis , pH and solubility of AH Plus, Sealer 26, Epiphany SE , Sealapex, Activ GP , MTA Fillapex ( MTA ‐ F ) and an experimental MTA ‐based Sealer ( MTA ‐ S ). Methodology Sealer samples were manipulated and stored for 2 or 7 days. Prepared sealers were evaluated by a modified direct contact test ( DCT ) for 5 h, 10 h or 15 h with biofilm previously induced on bovine dentine for 14 days. In the control group, the biofilm was not exposed to the sealers. The number of colony‐forming units ( CFU mL −1 ) in the remaining biofilm was determined. Sealer solubility was assessed by the percentage of mass loss after 15 h of immersion in distilled water. Sealer pH was measured at 5 h, 10 h and 15 h. Statistical analysis was performed using Kruskal–Wallis and Dunn or anova and Tamhane's T2 tests, at 5% significance. Results At 2 days post‐manipulation, the DCT showed that Sealapex and MTA ‐F were associated with a reduction in the number of bacteria in all 3 contact periods evaluated, compared with the control group ( P < 0.05). At 7 days, Sealapex had the greatest antibiofilm action at 10 h and 15 h. Sealapex had the highest pH values 2 and 7 days post‐manipulation. Regarding the solubility, at 2 days the highest values were observed for MTA ‐ F , MTA ‐ S , Sealapex and Activ GP ( P < 0.05). At 7 days, MTA ‐ S and MTA ‐ F had greater solubility than the other materials ( P < 0.05). AH Plus had the lowest solubility for both post‐manipulation periods ( P < 0.05). Conclusion Sealapex and MTA ‐ F were associated with a reduction in the number of bacteria in biofilms and had greater solubility. The high solubility and pH may be related to the antibacterial activity of these materials.