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The effect of calcium hydroxide on the antibiotic component of O dontopaste ® and L edermix ® paste
Author(s) -
Athanassiadis M.,
Jacobsen N.,
Nassery K.,
Parashos P.
Publication year - 2013
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/iej.12021
Subject(s) - calcium hydroxide , high performance liquid chromatography , calcium , chemistry , chromatography , nuclear chemistry , hydroxide , inorganic chemistry , organic chemistry
Aim To investigate the chemical interaction of calcium hydroxide with the antibiotics demeclocycline calcium in L edermix ® Paste and clindamycin hydrochloride in O dontopaste ® . Methodology Validated methods were developed to analyse the interaction of calcium hydroxide in two forms, P ulpdent ® and calcium hydroxide powder, with the two antibiotics. High‐performance liquid chromatography ( HPLC ) was used to analyse the mixed samples of the pastes and calcium hydroxide. The concentration of demeclocycline calcium over 0‐, 1‐, 18‐, 24‐, 72‐h and 7‐day time‐points was determined. The concentration of clindamycin hydrochloride over 1‐, 6‐, 24‐, 72‐h and 7‐day time‐points was determined. All tests with HPLC involved testing of the standard in duplicate alongside the samples. Linearity, precision and specificity of the testing procedures and apparatus were validated. Descriptive statistics are provided. Results The antibiotics in both O dontopaste ® and L edermix ® Paste were affected by the addition of calcium hydroxide. When mixed with calcium hydroxide powder, O dontopaste ® had a 2% loss of clindamycin hydrochloride over 7 days, but when mixed with P ulpdent ® , there was a 36% loss over 7 days. L edermix ® Paste showed an 80% loss of demeclocycline calcium over 7 days when mixed with calcium hydroxide powder and a 19% loss when mixed with P ulpdent ® over the 7‐day period. Conclusion The addition of calcium hydroxide to O dontopaste ® or L edermix ® Paste results in reductions of the respective antibiotic over a 7‐day time period.