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In vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota
Author(s) -
Raangs GC,
Winkel EG,
Winkelhoff AJ
Publication year - 2013
Publication title -
international journal of dental hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 38
eISSN - 1601-5037
pISSN - 1601-5029
DOI - 10.1111/idh.12014
Subject(s) - cetylpyridinium chloride , fusobacterium nucleatum , chlorhexidine , medicine , mouth rinse , antimicrobial , microbiology and biotechnology , aggregatibacter actinomycetemcomitans , prevotella intermedia , actinobacillus , porphyromonas gingivalis , dentistry , agar plate , tongue , chemistry , periodontitis , bacteria , biology , pathology , pulmonary surfactant , biochemistry , genetics
Objectives The aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride ( MR 1) with a stannous fluoride‐based mouth rinse ( MR 2) in vitro . Materials and methods Samples of the tongues from 10 subjects with and 10 subjects without halitosis were inoculated on blood agar plates. The agar was perforated, and the cylindrical holes were filled either with mouth rinse MR 1 or with mouth rinse MR 2. After incubation, inhibition zones of the whole tongue microbiota and Fusobacterium nucleatum were measured. In addition, MR 1 and MR 2 were applied in a short interval killing test ( SIKT ) on four oral pathogens Porphyromonas gingivalis , Prevotella intermedia , F. nucleatum and Aggregatibacter actinomycetemcomitans . Total viable cell counts were made after two minutes of incubation with increasing concentrations of MR 1 and MR 2. Results MR 1 showed a significantly higher in vitro antimicrobial activity against the whole tongue microbiota and F. nucleatum than MR 2 in both groups of subjects. In the SIK test, MR 1 showed a significantly greater killing capacity than MR 2. The results show that a mouth rinse with low concentrations of chlorhexidine and 0.05% cetylpyridinium chloride appears to be more effective in inhibiting growth of the human tongue microbiota in vitro than a fluoride/stannous fluoride‐containing mouth rinse. Conclusion This in vitro observation supports the use of chlorhexidine and cetylpyridinium chloride in the treatment of oral halitosis.