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Low‐level red plus near infrared lights combination induces expressions of collagen and elastin in human skin in vitro
Author(s) -
Li WenHwa,
Seo InSeok,
Kim Brian,
Fassih Ali,
Southall Michael D.,
Parsa Ramine
Publication year - 2021
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12698
Subject(s) - elastin , fibroblast , human skin , ageing , chemistry , in vitro , microbiology and biotechnology , procollagen peptidase , biochemistry , biophysics , pathology , medicine , biology , genetics
Objective Light therapy has attracted medical interests as a safe, alternative treatment for photo‐ageing and photo‐damaged skin. Recent research suggested the therapeutic activity of red and infrared (IR) lights may be effective at much lower energy levels than those used clinically. This study was to evaluate the efficacy of low‐level red plus near IR light emitting diode (LED) combination on collagen and elastin and ATP production. Methods Human dermal fibroblasts or skin tissues were irradiated daily by red (640 nm) plus near IR (830 nm) LED lights combination at 0.5 mW/cm 2 for 10 minutes (0.3 J/cm 2 ). qPCR, ELISAs or histology were used to determine the gene and protein expressions. Fluorescent measurement was used to assess crosslinks of collagen and elastic fibres. ATP production was evaluated by ATP assay. Results Treatment of human fibroblast cell cultures with low‐level red plus near IR lights combination was found to significantly increase LOXL1, ELN and COL1A1 and COL3A1 gene expressions as well as the synthesis of the procollagen type I and elastin proteins. Treating human skin explants with low‐level red plus near IR lights combination similarly induced significant increases in the same gene expressions, type III collagen and elastic fibre formation and crosslinks. ATP production was increased in human dermal fibroblasts after red plus near IR lights combination treatment. Conclusion Low‐level red plus near IR lights combination stimulated the production of collagen and elastin production associated with anti‐ageing benefits. These findings suggest that low‐level red plus near IR LED light combination may provide an effective treatment opportunity for people with photo‐aged skin.