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Sphingolipid identification and skin barrier recovery capacity of a milk sphingolipid‐enriched fraction (MSEF) from buttermilk powder
Author(s) -
Lee K.,
Kim S.,
Kim A.,
Suh H.J.,
Hong K.B.
Publication year - 2020
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12612
Subject(s) - sphingolipid , transepidermal water loss , chemistry , sphingomyelin , chromatography , lactosylceramide , ceramide , fraction (chemistry) , food science , biochemistry , medicine , stratum corneum , membrane , glycolipid , apoptosis , pathology
Objective In this study, we isolated the milk sphingolipid‐enriched fraction (MSEF) of sweet buttermilk powder and conducted a clinical trial for evaluating its efficacy in skin barrier recovery. Methods Milk sphingolipid‐enriched fraction was isolated via solvent extraction of buttermilk powder, and further concentrated by removing the phospholipids and neutral lipids. A cream containing 1% MSEF was used during clinical trials to assess for water holding and skin barrier recovery capacities. Results The main components of the MSEF were sphingomyelin, glucosylceramide and lactosylceramide, confirmed by TLC, HPLC, MS and NMR. The MSEF cream‐treated group had a significantly higher ( P < 0.05) water holding capacity, compared with the base cream (vehicle) group. Compared with that in the base cream group, transepidermal water loss (TEWL) recovery increased in the presence of the sphingolipid‐containing MSEF cream (MSEF group), with a significant difference ( P < 0.05) recorded on day 14. Conclusion The MSEF cream contributed to improving the water holding capacity and skin barrier recovery of damaged skin. Therefore, sphingolipid‐containing MSEF can be useful for strengthening or repairing skin barrier function.