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Exploring pathways for sustained melanogenesis in facial melasma: an immunofluorescence study
Author(s) -
Espósito A. C. C.,
Brianezi G.,
Souza N. P.,
Miot L. D. B.,
Marques M. E. A.,
Miot H. A.
Publication year - 2018
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12468
Subject(s) - melasma , dermis , epidermis (zoology) , melanocyte , photoaging , dermatology , wnt signaling pathway , immunofluorescence , human skin , medicine , cancer research , pathology , biology , microbiology and biotechnology , signal transduction , immunology , anatomy , antibody , melanoma , genetics
Background The physiopathology of epidermal hypermelanization in melasma is not completely understood. Several cytokines and growth factors are increased in skin with melasma, nevertheless, nor the pathways involved in the increased α MSH expression have been adequately evaluated, nor a model for sustained focal melanogenesis is available. Objective To explore stimulatory pathways for epidermal pigmentation in facial melasma related to α MSH : those linked to ultraviolet radiation, oxidative stress, inflammation, neural crest pigmentation cell differentiation and antagonism of α MSH . Methods Paired skin biopsies (3 mm) from 26 women with facial melasma and from normal adjacent skin (<2 cm far) were processed for immunofluorescence with markers for p53, p38, α MSH , MC 1R, Melan‐A, IL ‐1 α , COX 2, Wnt1, WIF ‐1 and ASIP . Results The fluorescence intensity in the skin from melasma was higher for MC 1R, α MSH at epidermis as at melanocytes ( P < 0.05). There were no differences between the sites in epidermal protein expression of COX 2, IL ‐1 α , p53, WIF ‐1 and ASIP ( P > 0.1). P53 was expressed only in epidermis, without difference between sites ( P = 0.92). WNT 1 was remarkable in the epidermis of melasma ( P < 0.01), but not in dermis. Positive p38 cells were prominent in the upper dermis of melasma ( P < 0.01), despite no marking in epidermis. Conclusion Melanogenesis in melasma involves epithelial secretion of α MSH and activation of the Wnt pathway; nevertheless, it seems to be independent of the stimulation by ultraviolet radiation/p53, IL ‐1 α , COX 2/PgE 2 , WIF ‐1 and ASIP . Damaged cells at upper dermis suggest the role of senescence/autophagy in sustained pigmentation in melasma.