Premium
Licoricidin, an isoflavonoid isolated from Glycyrrhiza uralensis Fisher, prevents UVA ‐induced photoaging of human dermal fibroblasts
Author(s) -
Kim K. J.,
Xuan S. H.,
Park S. N.
Publication year - 2017
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12357
Subject(s) - photoaging , glycyrrhiza uralensis , isoflavonoid , glycyrrhiza , traditional medicine , chemistry , biology , pharmacology , flavonoid , medicine , antioxidant , biochemistry , genetics , alternative medicine , pathology
Objective Licoricidin is an isoflavonoid isolated from Glycyrrhiza uralensis Fisher. In this study, we investigated the effects of licoricidin on photoaging of UVA ‐irradiated human dermal fibroblasts ( HDF s). Methods In vitro reactive oxygen species ( ROS ) scavenging activity, cellular protective effect and inhibition of elastase activity was determined by Fe 3+ ‐ EDTA /H 2 O 2 systems, photohaemolysis and elastase activity assay, respectively. Anti‐oxidative capacity of the compound was evaluated by fluorescent ELISA and 2′, 7′‐dichlorofluorescin‐diacetate ( DCF ‐ DA ) assay. The expression of protein and phosphorylation was examined using Western blot. Results The ROS scavenging activity ( OSC 50 ) of licoricidin was 2.77 μM. It was 3.1‐fold higher than that of L‐ascorbic acid. Its protective effects were confirmed in a study of 1 O 2 ‐induced cellular damage to human erythrocytes. The τ 50 value of 10 μM of licoricidin was 71.0 min; this was markedly higher than that obtained with α ‐tocopherol (37.0 min). The elastase inhibitory activity of licoricidin ( IC 50 of 61.2 μM) was 2.1‐fold more potent than that of oleanolic acid. Licoricidin markedly reduced the UVA ‐induced intracellular ROS in a concentration‐dependent manner. Western blot revealed that licoricidin attenuated the UVA ‐dependent induction of MMP ‐1 protein. Mechanistically, this appeared to be due to licoricidin‐dependent inhibition of mitogen‐activated protein kinases ( MAPK ) phosphorylation, which resulted in decreased c‐Jun activation and reduced c‐Jun and c‐Fos expression. Conclusion Licoricidin blocks UVA ‐induced photoaging via ROS scavenging. This activity converges to limit the activity of MMP ‐1. These data suggest that licoricidin may be considered as an active ingredient in new topically applied anti‐ageing formulations.