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Evaluation of anti‐microbial activities of ZnO, citric acid and a mixture of both against Propionibacterium acnes
Author(s) -
Bae J. Y.,
Park S. N.
Publication year - 2016
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/ics.12318
Subject(s) - propionibacterium acnes , citric acid , chemistry , active ingredient , food science , particle size , ingredient , microorganism , antimicrobial , microbiology and biotechnology , bacteria , nuclear chemistry , biology , organic chemistry , bioinformatics , genetics
Objective In this study, anti‐microbial activities of ZnO of three different particle sizes of citric acid ( CA ) and of mixtures of ZnO and CA were confirmed against Propionibacterium acnes . Methods ZnO with the smallest particle size showed relatively high anti‐microbial activity by disc diffusion assay and broth macrodilution assay. The mixtures of ZnO and CA also showed relatively high anti‐microbial activity when the particle size of ZnO was the smallest. Furthermore, anti‐microbial activities of ZnO, CA and the mixtures of ZnO and CA were compared through the checkerboard assay. Results The results indicated that a 1 : 1 ratio of ZnO and CA resulted in the highest anti‐microbial activity. The substances were confirmed to have synergic anti‐microbial effects. With the time‐kill curve assay, the mixture of ZnO‐containing CA reduced the surviving microbial content the most after 24 h. Conclusion The results of our study suggest that ZnO may not only be an anti‐microbial ingredient for the prevention of and treatment of acne. The results of our study suggest that ZnO may be an anti‐microbial ingredient for the prevention of and treatment of acne when mixed with CA .